What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM ...

Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...

Researchers used structured light and switchable fluorescent molecules to reduce the background light from the out-of-plane regions of microscope samples. This method allowed for the acquisition of ...

Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

Computational methods and algorithmic data processing are an important aspect of super-resolution microscopy, but a new approach to the task could offer a route to enhanced imaging. Generative AI is ...

Fluorescence microscopy reveals cellular morphology and dynamics in remarkable detail, but achieving clear visualization at fast acquisition rates remains a challenge. A fundamental trade-off between ...

SIM uses multiple images acquired under structured illumination to reconstruct a super-resolved image, exceeding the diffraction limit of traditional microscopy. However, SIM requires acquiring ...

Peking University, Aug 18, 2025: Professor Xi Peng’s team from the College of Future Technology at Peking University has recently developed a triangle structured illumination microscopy (SIM), which ...