In this guide, I will show you how to perform the delta-delta Ct method for calculating relative gene expression values in qPCR by using Microsoft Excel.

The 2-ΔΔCT method has been extensively used as a relative quantification strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This method is a convenient way to calculate …

Delta-Delta Ct method or Livak method is the most preferred method for qPCR data analysis. However, it can only be used when certain criteria are met. Please refer the lecture notes to make sure that …

Jan 21, 2026 · Double delta Ct analysis of qPCR data is one of the 2 most common methods of analysis. Here are the 4 steps to using this method.

With this data, you will work in groups and proceed to calculate the change in expression values for each gene in liver and brain tissue. The CT data is used to determine the amount of each …

Dec 1, 2001 · The 2 −ΔΔCT method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the …

With the ddCt package, one can acquire the relative expression of the target gene in di erent samples. This vignette mainly dicusses the principles of the ddCt algorithm and demonstrates the functionality …

Sep 13, 2025 · same samples, obtaining delta Ct (dCt) values. Subsequently, the dCt values are transformed using the 2-dCtformula, summarised by means in the compared study groups, and a …

The ΔΔCT method, also known as the Livak method, is a widely accepted approach for relative quantification of gene expression using qPCR data. It compares the CT (Cycle Threshold) values of …

The 2 (-Delta Delta C (T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the …