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Number of Polypeptide Chains in a Multisubunit Protein A sample (660 mg ) of an oligomeric protein of Mr 132,000 was treated with an excess of 1 -fluoro- 2,4 -dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. The peptide bonds of the protein were then completely hydrolyzed by heating it with concentrated HCl. The hydrolysate was found to contain 5.5 mg of the following compound: 2,4 -Dinitrophenyl derivatives of the α-amino groups of o
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